Seven-Color, Homogeneous Detection of Six PCR Products
نویسندگان
چکیده
منابع مشابه
Detection of asymmetric PCR products in homogeneous solution by fluorescence correlation spectroscopy.
The yield of the double-stranded DNA product (500 bp) of asymmetric PCR with a rhodamine-labeled primer (Rho-primer) was determined in a homogeneous solution using fluorescence correlation spectroscopy (FCS). FCS provides the average number of molecules in a focused volume and the diffusion constant that relates the molecular weight. Since FCS measures the fluctuation of fluorescence intensity ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99272rr01